FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_8721.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_8721.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21322667
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG159200.018.625751145
CTCCGAC50000.017.39982145
TCTCCGC150300.015.725054145
TATACTG237450.015.6643495
GAGACCG66150.015.343759145
TACATCG142100.015.254946145
TACACCG33450.014.7394055
CACGAGC224550.014.270617145
TGCCGTG104300.013.832552145
CTACACT121650.013.7678784
TATACCG28200.013.6268015
CGTCTTG103650.013.429675145
TACACTG166450.013.4163415
GTATAGA147150.013.2049751
GTATAGG78700.013.1742031
GTGTAGG88600.012.8478071
GAGCCCC178300.012.564368145
GTATAAG131000.012.5637031
GTGTAAG125600.012.353421
GTCCTAC60000.012.325711
Produced by FastQC (version 0.11.5)