FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_8230.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_8230.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20061753
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG75050.019.610086145
CTCCGAC61550.018.139563145
ATGCCGG126100.017.478037145
CACGAGC190550.016.056011145
GTATAGG61500.014.3830611
TCTCCGC155900.014.323158145
CGTCTTG76750.014.169255145
TAGACTG120000.013.7156245
GTATAAG87750.013.4681251
TACACTG140650.013.2999795
CTATACT95500.013.058554
GTGTAGG97350.013.03373051
CTAGACT107650.012.9990964
GTAAGAC102950.012.88820653
TCTGTCG99150.012.8694058
TATACTG118700.012.6441785
GTATTAG103200.012.5759261
TATACCG18450.012.575485
TACAGGT377900.012.4903324
CTACACT120600.012.3247014
Produced by FastQC (version 0.11.5)