FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_7431.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_7431.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20749754
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG62000.020.463465145
TACACCG64050.017.320385
TATACTG210300.015.41185
TACACTG135550.015.40558055
GAGCCCC147050.014.593501145
GTATAGG92800.014.5326511
CTATACT153550.014.3079334
GTGTAGG104300.013.764511
CACGAGC166250.013.475026145
TCTCCGC141150.013.405791145
TATACCG53700.013.23234755
TACACAG164950.013.2312025
GTATAGA158650.013.1623161
CTTACAC112450.013.0893343
TAGACAG194300.013.0611185
CTACACT116200.012.9790014
GTAGGAC70700.012.7169563
TCTATAC160600.012.641353
CTAACAC125050.012.6401943
GTGTAAG114250.012.6292281
Produced by FastQC (version 0.11.5)