FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_7379.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_7379.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22276469
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG176850.017.627756145
TCTCCGC177000.015.728655145
CTACACT129200.015.2642724
CCTACAC98900.015.1753893
GTATAGG89250.014.8664961
TATACTG226800.014.8335675
GAGACCG81850.014.792153145
TACACTG166450.014.7668065
CTATACT161200.014.7079594
GTATAGA154550.014.543151
TACATCG156350.014.328285145
TATACCG36350.013.763075
GAGCCCC185800.013.69604145
TACACCG46250.013.6388635
CACGAGC248250.013.521515145
CTCCGAC67050.013.515897145
GTATTAG151650.013.2913221
CGTCTTG120550.013.230891145
GTATAAG146000.013.2097491
CTAGGAC83050.012.8334513
Produced by FastQC (version 0.11.5)