FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_6215.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_6215.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23092727
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG182600.021.082743145
CGTCTTG109600.018.918594145
TCTCCGC175700.018.23828145
CTCCGAC54100.017.957294145
GAGACCG76250.017.494896145
TACACTG188750.016.095475
TATACTG268000.015.7187485
CTACACT139600.015.1661054
CCTACAC98750.014.97835353
TACATCG159850.014.830934145
CACGAGC269750.014.567036145
TGCCGTG118550.014.554885145
GTATAGG82300.013.8315181
CTTACAC133600.013.5676483
TATACCG21550.013.4582735
CTATACT162900.013.4419574
GTCCTAG98200.013.2163521
CTAGACT116800.013.1604184
GTGTAGG97700.012.9871411
CCTATAC81150.012.8660413
Produced by FastQC (version 0.11.5)