FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_6214.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_6214.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22972154
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG61150.027.624357145
CTCCGAC41500.023.409353145
TCTCCGC142250.019.31611145
TATACTG244300.016.501375
CACGAGC118300.016.301577145
ATGCCGG305150.015.823173145
CCACGAC54600.015.668307145
TACACTG192200.015.1272375
TGCCGTG186700.014.67845145
GTGTAGG99850.014.4500361
GAGCCCC213650.014.184241145
GTATAGA157350.013.9617481
TCTCGTT116650.013.859668145
CTACACT140300.013.7981344
CGTACAC21800.013.6361343
GTCCTAG102700.013.20185851
TCTGTCG73850.013.1550858
CGTCTTG171300.012.866159145
CTATACT159500.012.8644884
TAACACG56350.012.7381994
Produced by FastQC (version 0.11.5)