FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_5867.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_5867.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18822034
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG203600.016.4169315
TACACTG138750.015.4155075
TACACCG51600.014.3324115
CTACACT109650.014.2828184
CTATACT131550.013.50342754
GTATAGG75900.013.4691561
GTGTAAG111300.013.2891751
CCTACAC90650.013.1171733
CCTATAC77500.013.0975643
TATACCG33350.013.0444035
TAGACAG213800.012.9885265
GTGTAGG90900.012.6822491
TCTACAC119750.012.6542083
GTATTAG137100.012.3748931
CTAGGAC69400.012.3278333
TACACAG175150.012.2532235
GTATAGA138650.012.2365511
ATACTGT269950.012.2207226
TCTCCGC112150.012.153228145
TCTATAC134300.012.093063
Produced by FastQC (version 0.11.5)