FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_4340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_4340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18463380
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG148000.016.3628085
TACACTG89750.015.7533635
CTACACT93400.015.6034684
TAGATCG77750.015.292433145
CTATACT137200.014.7442284
TATACCG69150.014.4697165
TCTACAC119950.014.2651993
GAGCCCC100200.013.892059145
GTATAGG91850.013.5772871
TATACAG151950.013.0744365
TACACCG88250.012.9812185
GTATAGA145800.012.8797061
TTATACT230150.012.7904854
TAGACAG107700.012.7238695
GTATAAG128000.012.5749641
TCTATAC148000.012.541353
GTGTATA120450.012.5204581
CTAGGAC40550.012.3374183
TAGGACT88600.012.2752254
CTTACAC87900.012.2078423
Produced by FastQC (version 0.11.5)