FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_3256.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_3256.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23846978
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG56700.030.176048145
CTCCGAC50600.021.635147145
TATACTG255650.016.7901615
TCTCCGC167400.016.760576145
CACGAGC192750.016.737827145
CTATACT158850.015.0170914
TATACCG27350.014.8460065
TACACTG189000.014.7315485
CCACGAC66100.014.0391865145
TACACCG30550.014.0029565
TCTATAC158050.013.9001313
CTACACT141400.013.7423994
GAGCCCC207900.013.635017145
GTATAGA159200.013.6173751
CCTACAC102050.013.5703533
GTATTAG161300.013.0804871
CTAGTAC68050.012.466043
TAGACAG298200.012.4491965
GTGTAAG146500.012.4222821
TACACAG233800.012.3739215
Produced by FastQC (version 0.11.5)