FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_30982.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_30982.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17557528
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC70700.015.99691145
TATACTG185000.015.9513745
TACACTG128300.015.7106295
CCTACAC88000.014.5010833
CTATACT131050.014.1084254
CCTATAC75750.013.7832093
CTACACT98250.013.6525764
GTATAGG72300.013.5383181
GTATTAG125000.013.5149731
TACACCG51150.013.4664575
TATACAG158500.013.1746375
TAGACAG195250.013.1086845
GTATAGA128350.013.1057341
TCTATAC130400.012.9553393
CACGAGC113850.012.926886145
TAGACTG100300.012.795225
TACACAG154500.012.6709945
CTAGACT72850.012.5405454
GTGTAAG106000.012.4490081
GTACTAG53150.012.4138751
Produced by FastQC (version 0.11.5)