FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_30978.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_30978.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20662579
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGC81900.026.467865145
CTCCGAC38350.021.929274145
ACCGGAC24850.020.13049145
ATGCCGG125900.018.542221145
CACGAGC139200.017.968485145
TCTCCGC113950.016.5421145
TATACTG220850.016.1201215
CGTCTTG81500.016.10099145
TGCCGTG87000.015.749767145
TACACCG22350.014.92328555
TACACTG181300.014.7175045
CCTATCG112300.014.461051145
CCTATAC81200.013.8405893
CTATACT150700.013.5680984
GTATAGG78900.013.5087791
CTACACT128500.013.3729844
GTACTAG64050.013.244711
TATACCG18750.013.1480645
CCTACAC91600.012.9024163
GTGTAGG87350.012.866031
Produced by FastQC (version 0.11.5)