FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_30978.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_30978.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21145026
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG111150.021.655231145
AGACGGC123350.019.98361145
ACGGACG26000.018.124842145
CTCCGAC34050.017.459465145
CCTATCG99750.016.42592145
TATACTG223250.015.9464245
CACGAGC69450.015.763002145
TCTCCGC98250.015.643629145
TACACTG178450.015.399125
CCTACAC93250.015.0063673
GTGTAGG94350.014.6776141
CGTCTTG79150.014.655589145
CTTACAC134200.014.1551953
GTACTAG68400.014.0980661
CTACACT134800.014.03856854
CTATACT153700.013.3972824
TCTATAC150650.013.2352043
TGCCGTG88300.013.219024145
TACACCG21650.013.0611075
GTATTAG146800.012.9895371
Produced by FastQC (version 0.11.5)