FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_30976.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_30976.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18292291
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG47500.021.826073145
TCTCCGC71350.017.37542145
CTCCGAC24800.016.66312145
TATACTG240950.015.8284455
CACGAGC52550.015.451778145
TACACTG164050.014.5411625
GTGTAGG80650.014.4740171
TCTATAC136100.013.8512063
CTATACT143850.013.81084254
GTATAGA138250.013.5832031
CTACACT115500.013.5597364
GTACGAC8352.5411282E-913.02498153
TACACCG16850.012.9092445
ATACTGT304400.012.8626296
TAGACAG266650.012.8073365
GTATAGG71050.012.7559711
GTGTAAG114500.012.72794151
TACACAG206050.012.7032275
ATGATCG51900.012.572113145
CTAGTAC61350.012.5274773
Produced by FastQC (version 0.11.5)