FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_30908.TR4_batch_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_30908.TR4_batch_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10491834
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG44100.021.20728145
TCTCCGC52200.017.222055145
CTCCGAC21150.017.139317145
TATACTG126350.016.8133875
GTATTAG71200.015.9873431
GTCCTAC28900.015.5542991
TCTATAC74300.014.9300513
CCTACAC49550.014.7787063
TACACTG90500.014.6610595
TACACAG108600.014.5542375
CTATACT79000.014.500764
GTGTAGA62800.014.431351
TCTACAC65400.014.4120023
CTTACAC66050.014.3799433
CGTCTTG30000.014.258198145
CTACACT68450.014.1935854
GTACTAG30700.014.1699881
TACACCG11800.014.1321625
ATACTGT163250.013.9901476
GTACGGA11000.013.8415021
Produced by FastQC (version 0.11.5)