FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_30903.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_30903.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20093249
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG61150.017.90253145
TATACTG217300.016.3493945
TACACTG152350.015.0862775
CTATACT141100.014.6961374
TCTCCGC130450.014.672151145
GTATTAG141450.013.4806051
ATGCCGG233050.013.407941145
TCTATAC142050.013.1685613
GTATAGA146800.013.1374851
GTGTAGG91250.013.0306951
CTACACT115800.012.9606264
TACACCG49700.012.8378285
CTAGTAC60600.012.80183
ATACTGT290200.012.5671226
GTGTAAG114850.012.5625751
TACACAG187250.012.4293515
TAGGACT109500.012.3820254
CTTACAC111400.012.36597253
GTATAGG76950.012.1545171
TAGACAG226300.012.0146745
Produced by FastQC (version 0.11.5)