FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_30889.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_30889.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18391333
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG36600.030.307035145
TATACTG185000.015.5594565
TACACCG38750.015.1561525
TACACTG134500.014.8247045
GTATAGG80650.014.65385151
GTATAGA135600.013.9021561
CTACACT104750.013.7744634
GTGTAGG88650.013.7403911
TCTCCGC102850.013.463631145
CACGAGC125150.013.092141145
CTATACT139600.012.984654
CTCCGAC39650.012.9822145
GTATAAG131300.012.8112581
GTCTAGG71800.012.7237351
CCTAGAC74300.012.3933343
TAATACT221450.012.3108434
GTATTAG147700.012.2723591
CCTACAC84750.012.2340333
GTGTAAG111850.012.0571731
TAGTACT105500.012.0271084
Produced by FastQC (version 0.11.5)