FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_30887.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_30887.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22239187
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG40050.021.360603145
AGACGGC198800.019.802383145
CTCCGAC49300.018.676298145
TCTCCGC148750.018.42336145
ATGCCGG192150.017.997492145
CACGAGC109600.015.809617145
CCACGAC64700.015.239427145
CTATACT150500.015.0310124
CGTCTTG120000.014.801946145
TACACTG181100.014.6933785
GTATAGG80750.014.4558861
TGCCGTG123600.014.42948145
TATACTG233700.014.3646935
CCTATCG170300.013.878322145
CCTATAC84250.013.5112033
GTATTAG144500.013.1962111
GTATAGA143850.012.852621
CCTACAC100600.012.7567363
TCTATAC153350.012.6711293
CTTACAC125650.012.521653
Produced by FastQC (version 0.11.5)