FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_30852.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_30852.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21600567
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG166750.019.478031145
GAGACCG72200.017.070436145
CTCCGAC56700.016.238787145
TCTCCGC167800.016.11572145
TATACTG224800.016.0300255
CGTCTTG105200.015.988405145
TGCCGTG103650.015.737876145
CACGAGC247650.015.2521925145
TACACTG167500.015.19385
TACATCG150600.014.490201145
CCTACAC94400.014.1324323
GTATAGG81100.013.9467751
CTAGGAC88950.013.8571563
TAGGACT122700.013.709344
CTACACT126350.013.6576144
TATACCG25200.013.5229495
GAGCCCC188000.013.458618145
GTATAGA143400.013.1965821
TACACAG204950.013.1957795
CTATACT149250.013.0194784
Produced by FastQC (version 0.11.5)