FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_30852.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_30852.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21562145
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG173550.016.3766485
AGACGGC298350.016.135239145
ATGCCGG259150.016.114048145
CTATACT134450.015.2611124
GAGCCCC146150.014.336145145
TCTCCGC207400.014.08736145
TACACCG83700.013.8598365
CTACACT106650.013.7325474
GTATTAG153650.013.6370941
GTGTAGG101050.013.5606971
TACACTG128750.013.3464175
GTATAGA151650.013.0041811
TAGTACT104800.012.8680344
TAGACAG158300.012.6412995
CCTACAC103550.012.4631093
TAGACCG39650.012.4345275
TGCCGTG166500.012.409777145
GTGTAAG113050.012.3777931
GTATAGG93750.012.373861
TCTATAC153250.012.3479983
Produced by FastQC (version 0.11.5)