FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_2397.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_2397.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20232727
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC38500.020.149246145
TCTCCGC107650.017.577707145
TATACTG233950.015.6815885
CACGAGC79550.014.490811145
TACACCG26950.014.2586925
TACACTG173600.014.2001125
TAGGACT118550.013.6996044
CCTATAC78800.013.6174893
CTAGGAC89850.013.5566613
GTACTAG65750.013.3428611
CTACACT124250.013.1878374
CTAACAC145850.013.0740713
GTGTAGG88850.012.893151
CTATACT146300.012.885184
TAACACG49700.012.8377174
TGCCGTG165050.012.431018145
TACACAG202000.012.4190215
GTATAGA143200.012.0501751
TATACAG186550.012.0095245
ACTGATC128550.011.9564068
Produced by FastQC (version 0.11.5)