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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2020-01-31, 20:01 based on data in: /beegfs/mk5636/logs/html/HV2J2BGXC/merged

        General Statistics

        Showing 10/10 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HV2J2BGXC_n01_1657_1_tninsertions2
        16.0%
        43%
        11.2
        HV2J2BGXC_n01_1657_2_tninsertions2
        8.0%
        45%
        13.7
        HV2J2BGXC_n01_1728_tninsertions2
        12.4%
        42%
        16.2
        HV2J2BGXC_n01_1734_tninsertions2
        11.2%
        45%
        9.6
        HV2J2BGXC_n01_1736_tninsertions2
        14.1%
        43%
        9.0
        HV2J2BGXC_n01_1740_tninsertions2
        18.2%
        43%
        12.3
        HV2J2BGXC_n01_1744_tninsertions2
        12.4%
        43%
        9.4
        HV2J2BGXC_n01_1747_tninsertions2
        10.4%
        44%
        9.9
        HV2J2BGXC_n01_1751_tninsertions2
        8.8%
        44%
        14.5
        HV2J2BGXC_n01_undetermined
        3.3%
        54%
        16.1

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 10/10 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        16,080,564
        13.2
        1657_1_tninsertions2
        11,216,139
        9.2
        1657_2_tninsertions2
        13,727,402
        11.2
        1728_tninsertions2
        16,216,798
        13.3
        1734_tninsertions2
        9,595,923
        7.9
        1736_tninsertions2
        9,031,540
        7.4
        1740_tninsertions2
        12,345,064
        10.1
        1744_tninsertions2
        9,427,812
        7.7
        1747_tninsertions2
        9,891,870
        8.1
        1751_tninsertions2
        14,520,341
        11.9

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        GGGGGGGG
        9498386.0
        59.1
        CCCCCCCC
        1858957.0
        11.6
        GCTAGCTA
        97231.0
        0.6
        AGAGAGGA
        83876.0
        0.5
        CGGGGGGG
        82470.0
        0.5
        GAGGCTGA
        64702.0
        0.4
        GTACTAGA
        56219.0
        0.3
        GCGGGGGG
        53108.0
        0.3
        AGCAGAAA
        48817.0
        0.3
        CGAGCTGA
        34759.0
        0.2
        TAGCATGA
        34454.0
        0.2
        GGGGGGTG
        33834.0
        0.2
        TAGGCGAA
        33297.0
        0.2
        TAAGCGAA
        32771.0
        0.2
        TCCTGACA
        32761.0
        0.2
        GTACGCTA
        32058.0
        0.2
        CGTATAGA
        30265.0
        0.2
        GTGGGGGG
        29415.0
        0.2
        GGGGGCGG
        27346.0
        0.2
        TCTCTACA
        24852.0
        0.1

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        4.0
        608,241,712
        122,053,453
        13.2
        0.9

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (151bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        10 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.