FastQCFastQC Report
Fri 31 Jan 2020
HV2J2BGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2J2BGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16080564
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATAA2829800.0140.79217
TGTCATA717150.0139.175896
GTTCATA682500.0137.201896
ATTCATA757750.0134.579656
TTTCATA1082250.0132.753226
AGTCATA516200.0132.611376
TCATAAG9356550.0126.695358
CGGTCAT105450.0126.5704965
TATCATA909550.0124.880326
GATCATA478650.0123.76336
GTCATAA2333150.0123.539477
GGTCATA621200.0122.777576
GTGTCAT189700.0122.3335
ATGTCAT217200.0121.697635
CGTTCAT137050.0121.6144565
CTTTCAT185950.0121.525085
GCTCATA340800.0120.832426
GGTTCAT182850.0120.532425
CCTCATA244050.0119.6292956
TATTCAT302900.0119.481475
Produced by FastQC (version 0.11.5)