FastQCFastQC Report
Fri 31 Jan 2020
HV2J2BGXC_n01_1751_tninsertions2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2J2BGXC_n01_1751_tninsertions2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14520341
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCATA1217450.0140.176336
GGTCATA924150.0140.145746
TTCATAA4443750.0139.623767
TTTCATA1640400.0139.058436
GTTCATA1124650.0138.78616
TATCATA1145300.0134.973466
AGTCATA806300.0132.154196
CGGTCAT163900.0132.12175
ATTCATA1061300.0131.60516
TCTCATA831050.0129.61426
TTGTCAT451250.0129.409525
GTGTCAT321650.0128.900445
CTTCATA760300.0127.801626
GTTTCAT432900.0127.760885
CGTTCAT216550.0127.182975
GGGGTCA71850.0127.133974
AGGTCAT248000.0126.4306265
ATGTCAT338500.0126.16775
TGTTCAT440550.0125.0980455
AAGAGAG373850.0125.06744145