FastQCFastQC Report
Fri 31 Jan 2020
HV2J2BGXC_n01_1747_tninsertions2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2J2BGXC_n01_1747_tninsertions2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9891870
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCATA1092350.0141.964086
TGTCATA821950.0141.495486
GGTCATA640400.0141.15946
GTTCATA751500.0141.06116
TTCATAA2999150.0139.8517
TATCATA765350.0138.110576
GTTTCAT276150.0134.546975
AGTCATA539950.0133.893086
CGGTCAT112300.0133.762715
GTGTCAT213650.0133.153065
CGTTCAT136100.0133.010365
TTGTCAT299000.0132.87235
AGGTCAT163300.0132.165375
ATTCATA711900.0132.114626
ATTTCAT286350.0130.944235
TTTTCAT414250.0130.924885
TCTCATA561950.0130.573556
GGTTCAT203650.0130.29325
CTTTCAT204050.0130.17995
TGTTCAT284650.0129.765145