FastQCFastQC Report
Fri 31 Jan 2020
HV2J2BGXC_n01_1744_tninsertions2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2J2BGXC_n01_1744_tninsertions2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9427812
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATAA2853600.0140.342967
TGTCATA787450.0140.00556
GGTCATA613950.0139.95336
TTTCATA1068250.0137.618476
GTTCATA741500.0136.499136
TATCATA735400.0135.492136
ATTCATA675600.0133.309596
AGTCATA517850.0133.16576
CGGTCAT112550.0131.210945
TCTCATA534350.0129.813496
CTTCATA486000.0128.795386
GTCATAA2457100.0127.692857
GTGTCAT218200.0125.8909155
GCTCATA356200.0125.578636
TTGTCAT302050.0125.337945
GTTTCAT284050.0124.5261465
CGTTCAT141750.0124.077275
AGGTCAT167650.0123.589955
CCTCATA241150.0123.5603266
TGTTCAT293200.0122.766475