FastQCFastQC Report
Fri 31 Jan 2020
HV2J2BGXC_n01_1740_tninsertions2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2J2BGXC_n01_1740_tninsertions2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12345064
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATAA3674650.0142.392567
GTCATAA2873700.0141.705117
TGTCATA1010850.0141.481726
TCATAAG10860050.0141.332588
GGTCATA793250.0140.170416
AGTCATA650950.0139.872856
TTTCATA1378400.0139.352486
GATCATA569650.0138.836266
ATTCATA854500.0138.581916
TATCATA960350.0137.658256
GTTCATA957650.0137.327186
CTTCATA597300.0136.778826
ATCATAA2637150.0136.23357
TAATAAG772750.0135.96028
CTCATAA1847950.0135.71277
TCTCATA683550.0134.22026
TCAGAAG411300.0133.837318
AAGAGAG629000.0133.36346145
AGGTCAT205650.0131.775025
GTGTCAT266900.0131.0065