FastQCFastQC Report
Fri 31 Jan 2020
HV2J2BGXC_n01_1734_tninsertions2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2J2BGXC_n01_1734_tninsertions2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9595923
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATAA2879350.0141.087427
GGTCATA623100.0141.025096
TTTCATA1052500.0140.832186
TGTCATA802400.0140.782266
GTTCATA743450.0139.775576
TATCATA736550.0137.226656
ATTCATA673150.0135.762916
GTGTCAT207650.0132.877825
CGGTCAT114500.0132.519385
CTTCATA480900.0131.409856
AGGTCAT163150.0131.350895
GTTTCAT273800.0130.932825
CGTTCAT142450.0130.386415
CCTATAG309600.0130.20442145
AGTCATA539650.0129.718146
TTGTCAT294200.0129.689965
TCTCATA545450.0128.843876
TGGTCAT219200.0128.588275
TGTTCAT285500.0127.700095
ATGTCAT221150.0127.388885
Produced by FastQC (version 0.11.5)