FastQCFastQC Report
Fri 31 Jan 2020
HV2J2BGXC_n01_1728_tninsertions2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2J2BGXC_n01_1728_tninsertions2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16216798
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATAA4861800.0141.985027
TGTCATA1337550.0140.651156
TCATAAG14372900.0140.269848
GTCATAA3846700.0139.581857
GGTCATA1079050.0138.730976
TTTCATA1823600.0137.960076
TATCATA1246350.0136.674776
GTTCATA1293300.0136.556326
AGTCATA862450.0136.376636
GATCATA759000.0136.243416
ATCATAA3453450.0135.140887
TAATAAG1005750.0135.132698
ATTCATA1147700.0134.70286
TCTCATA910000.0132.716066
AAGAGAG873350.0132.24347145
CTCATAA2505850.0131.487737
CTTCATA823150.0131.385546
GCTCATA612650.0130.20116
TCAGAAG569400.0129.918698
GTGTCAT368300.0128.162385