FastQCFastQC Report
Fri 31 Jan 2020
HV2J2BGXC_n01_1657_1_tninsertions2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2J2BGXC_n01_1657_1_tninsertions2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11216139
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATAA3373200.0141.72727
GGTCATA737550.0140.22616
TCATAAG10023250.0138.893078
TGTCATA954500.0138.879186
GTTCATA882400.0138.445336
GTCATAA2703000.0138.165397
TTTCATA1268700.0137.907686
AGTCATA591100.0136.935446
ATTCATA789800.0135.447076
TATCATA881450.0135.140176
GATCATA538450.0132.727696
TCTCATA622750.0132.420676
CTTCATA565600.0131.67586
TAATAAG706900.0131.446328
CTCATAA1685150.0131.300387
ATCATAA2480050.0130.857387
CCTCATA270150.0130.502086
CGGTCAT140250.0130.311945
GTGTCAT255950.0129.442025
TCAGAAG400900.0129.315548
Produced by FastQC (version 0.11.5)