FastQCFastQC Report
Tue 31 Oct 2017
HV2CCAFXX_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2CCAFXX_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72231521
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGGCAT1232638517.065105136025032No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31961824.424913051464055No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGCATCCGCCGTGGGCGGT15316312.1204468337306643No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC13063671.8085829869206271No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGACCAG9336351.2925589646658555No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATAAAGGCG6091300.8433021921274508No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGA5331040.7380489744913443No Hit
GGCTACCTTGTTACGACTTCACCCCAATCACCAGTTTTACCTTCGGCGGC4301390.595500404871718No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGA3900200.5399581714470612No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGGCCCTACCGTAGACGGC3774400.5225419522870077No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCACAGACCACACCGTGGTAATC3357870.4648759922970471No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAG3071640.4252492481779527No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTCGACAAC2846220.39404126627764074No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCACAAACCACACCGTGGTAATC2798450.38742781008307997No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCTTAGACGGC2600390.36000764818450937No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTGACCCTACCTTCGGCCGC1576930.21831604515153433No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA1493090.20670892421052575No Hit
GGAAACCTTGTTACGACTTGTACTTCCTCTAAATGACCAAGTTTGTACAG1307730.18104699747358222No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGAA1121200.15522309159182734No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT1054290.14595982272060973No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCTGTCCCGCCTTAGGCGGC988860.1369014505453928No Hit
AGATACCTTGTTACGACTTAACCCTCCTTGCTAAATCTCAGTTCGACAAC969870.1342724044257631No Hit
GGCTACCTTGTTACGACTTCACCCTAATCATCTGTCCTACCTTAGACGGC906010.12543138888076302No Hit
GAAAACCTTGTTACGACTTTTGCCCGGTTCAAACAACAGCGATTGGAAGG837420.11593553457084199No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTGACCCTACCTTAGGCCGC818610.11333140831964483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTGT23923600.093.971515
CCTTGTT23958250.093.971326
TGTTACG24034400.093.952419
CTTGTTA23939550.093.94747
AAACCTT17318250.093.9408953
GTTACCT4007150.093.422152
ACCGTGT133600.093.234825
GGCTACC2108450.093.045921
CCTGAGG1181250.092.9167894
GATACCT500900.092.8528062
TTGTTAC24201950.092.8210458
AGATACC461550.092.6846161
CTCATGG1098950.092.6047994
CCGGAAG340000.092.215894
GCCTTGT66600.091.538645
GAAACCT17713250.091.4703752
AACCTTG17831850.091.3781364
GTCCCCG249300.091.1711394
GGAAACC17892600.090.375421
GATACCG97850.089.515942
Produced by FastQC (version 0.11.2)