FastQCFastQC Report
Tue 31 Oct 2017
HV2CCAFXX_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2CCAFXX_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72231521
Sequences flagged as poor quality0
Sequence length12
%GC82

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGG4707281665.1693545259832No Hit
CTTATCAAGGCG55763997.720173855954106No Hit
TAATGACCACGC38503045.330503839175697No Hit
TGCCTACAAGCA25491553.5291448452262273No Hit
TCCGACACAATT23494793.2527059758301364No Hit
CAGGGTGATACC16534992.2891654185158306No Hit
ATGGCAGCTCTA5681120.7865153497183037No Hit
ACCGTGCATAGG5558350.7695186150101976No Hit
CCAGTGTATGCA5287020.7319546822224607No Hit
CTAACCTCCGCT3267880.45241744251792787No Hit
TCCCATTGAAGG3167030.43845539401004724No Hit
CAGTAACGGCCA2234590.30936493778111085No Hit
AGCGACTGTGCA2113000.29253156665495106No Hit
AATGGCCTCTAT2029360.2809521344566453No Hit
TATCGTTGACCA2021660.279886117862588No Hit
ATGGGCTCTGAT1923370.26627848526130304No Hit
TGACGGACATCT1819340.25187618574444803No Hit
ACACCTGGTGAT1770150.24506613947669745No Hit
CCACGAAGCTAT1690220.2340003334555284No Hit
TCGTTTCGGGAA1601730.22174944924668No Hit
AATCCGTACAGC1535300.21255263335794908No Hit
TCGACCAGCAAT1377930.19076574616226066No Hit
GACTTTCCCTCG1334900.1848085131697559No Hit
CTTGATGCGTAT1313760.18188181306607126No Hit
AGTTTCTCTAGG1291530.1788042093146564No Hit
GCCTTCTAAGGA1249970.17305048858101715No Hit
AAGTGTTTGCCG1242440.1720080074182572No Hit
CATTCGATGACT1175670.16276412066693155No Hit
ATTGCAGGTACC1152300.15952869108211082No Hit
CGCAGCGGTATA1143180.1582660844148637No Hit
CAACCTTTACAG1102920.15269234050879255No Hit
ACCCATGACGGA1097360.15192259346165504No Hit
TTCAGACTACCA1070020.148137542334184No Hit
CGTGATCTCTCC1023180.14165283879319115No Hit
AATTTGATCCCG1000760.13854893073620864No Hit
CGAGGCTCAGTA996450.13795223833096357No Hit
GATTGCCGCAAG971740.13453129417003415No Hit
ACAGGAACTCGC954350.13212375799202677No Hit
GACAAGGCTCCA866240.11992548239431369No Hit
ACACGTAAGCCT784550.1086160154373601No Hit
ATGTGCACGACT783190.10842773198698115No Hit
AGGATCCGCATG729440.10098638238560696No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCG54950.06.0003741
CTTATGA2750.06.0003741
TAGAGTA35250.06.0003741
ATGGGAG33850.06.00037341
TCCCATG1101.813969E-76.00037341
ACGCTTT1405.18412E-106.00037341
GCCTTTC1054.8271795E-76.00037341
AATCCGT158600.06.00037341
TCCCAGT909.145993E-66.00037341
AATCTGT1800.06.00037341
TCCCACA1101.813969E-76.00037341
GTGTTCC4800.06.00037341
GCCTTCT126300.06.00037341
CAGTTAC13250.06.00037341
GTCATAT41650.06.00037341
TAGTGAC1054.8271795E-76.00037341
GATATCA550.0091559096.00037341
AGGATCC77850.06.00037341
TATCGTG650.00126123396.00037341
AAGAGCG1001.2856635E-66.00037341
Produced by FastQC (version 0.11.2)