FastQCFastQC Report
Tue 31 Oct 2017
HV2CCAFXX_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2CCAFXX_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72231521
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGGGGGCGAGG1210697316.761343015329828No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35453014.908246359646781No Hit
ACACCATGGGAGTTGTATTCGCCTTAAGTCGGGATACTAAATTGGTTACC15402542.132384835146971No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG13435091.860003750993974No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGGA9262961.2823985805310676No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACTGCTGTTTCGAA5160170.7143930971632177No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGGG4398230.6089072940884077No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATTGCGGCGACGTG3688940.5107105525301067No Hit
ACACCATGGGAGCGGATTGCACCAGAAGTAGTTAGCTTAACCGAAAGGAG3459850.47899448220119856No Hit
ACACCACGAAAGTCGGTTTTACCCGAAGCCGGTGAGCCAACTAGCAATAG2992260.4142595861992162No Hit
ACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAG2922440.4045934461216731No Hit
GCTACTACCGATCGAATGGCTTAGTGAGGCTTCCGGATTGTTGATGCGGA2759220.3819966632019281No Hit
ACACCATGAGAGTTTGTAACACCCAAAGCCGGTGGGGTAACCTTTTGGAG2665350.36900095181437476No Hit
AAACCATCCGAGTTGGGTTCCAGTGAGGCTGCCTCTAATTAGGGTTGTCG2359130.32660671786213663No Hit
CCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGGGGGCGAGG2034850.28171219044383683No Hit
ACACCATGAGAGTTGGCAATACCCGAAGTCCGTGAGCTAACCGCAAGGAG1553870.21512353311790292No Hit
ACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGTAAGGAG1485720.20568859404192802No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA1430860.19809357191855342No Hit
GTTACTACCGATTGAATGGCTTAGTGAGGCCTCCGGATGCGAATTCACTT1318320.1825131163996948No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGTG1305030.18067319944709456No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACTGCTGTTTCGAT1039730.1439440822518468No Hit
ACACCATGGGAGTTTGTAACGCCCAAAGTCGGTGGCCTAACCTTTATGGA994580.13769334858669252No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCTGCAAAGG988990.1369194482281496No Hit
AAACCACCCGAGTTGGGTTTCAGTGAGGCTGCCTCTTTTTGGGGTTGTCG978460.13546163592484783No Hit
GCTCCTACCGATTGAATGATCCGGTGAAATGTTCGGATCGCGGCGACGTG947560.13118372517726715No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGG920350.1274166717325529No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCTGAAAGGG916450.12687674124984852No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCCGCAAGGG913380.12645171904936073No Hit
ACACCATGAGAGTCTGTAACACCCAAAGCCGGTGGGATAACCTTTATAGG912930.12638941937827947No Hit
GCTGCCCGGAACTGAGCAATATCGAGAGGCAGGAAGAGATATAAATAATT857970.11878055288355342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAC13901700.093.966762
CTAGTAC1275650.093.963072
CCATGGG4243200.093.9402164
ACCATGG4225050.093.9322363
CGATTGA16234200.093.925719
TACTACC14068650.093.924163
ACTACCG14084250.093.9148254
CCGATTG16473000.093.9006968
GCTAGTA1274900.093.891681
CATGGGA4240650.093.871535
TGGGAGT3530350.093.871317
ATGGGAG4251500.093.748116
CTCCTAC1361550.093.741242
AGTACCG1287750.093.6996464
CGGGACT692050.093.6855247
CCGATCG340000.093.576198
CATGAGA1440800.093.555355
CGATCGA340100.093.5486769
ATGAGAG1443700.093.546546
TCCTACC1364300.093.544253
Produced by FastQC (version 0.11.2)