FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_shrt_15g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_shrt_15g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12305935
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG136350.11080019519036952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG85450.032.946655
TACTCTA60350.032.528814
ACTACCC74900.031.875536
CTAGTTG62750.030.4577228
CTGTTAC79350.030.1891211
TACTACC83050.028.7059275
TACCCTA82850.028.564928
TCTAGTT72000.028.5092547
CTCTAGT72550.027.772096
CTACCCT87300.027.2274137
GTTACTA88650.026.7370153
GTACTCT72950.026.5794473
TTACTAC95800.024.9935024
CCTACAC97700.024.40061611
ACCTCTA76400.024.11514717
CTAATGC75800.024.0773721
GTTACCT76400.024.06959514
GGACACC73000.023.34685529
TGTTACC80300.023.24421513
TGTTACT103150.023.2127692
Produced by FastQC (version 0.11.2)