FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_shrt_08r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_shrt_08r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16821716
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC425530.2529646797033073No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT424320.2522453713996836No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC381250.22664156260871365No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT304120.18079011677524456No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT230190.13684097389350763No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCGTC210310.12502291680587166No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTG193740.11517255433393359No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT192990.11472670207962136No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA181530.10791407963373059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT81000.038.7395441
ACTCTAG119100.028.0710775
GACTCTA116950.027.8497584
CTGACTC118350.026.6755122
CTAGCAG124950.024.9871568
TGACTCT151500.021.9996323
CTCTAGC147400.021.8857366
TAGCAGA156300.020.2181289
GTGCGCA170250.017.85321868
GGTGCGC173300.017.59862767
TCTAGCA355700.017.525687
TGCGCAT175050.017.40298369
AGGTGCG181900.016.7656466
CTATCGA197300.015.80794917
TCTATCG200250.015.59220916
AGGGGGG6900.015.5010914
GCAGATC210350.014.6955811
AAGGTGC210000.014.67017665
AGCAGAT217400.014.551846510
GATCTAT217200.014.24829614
Produced by FastQC (version 0.11.2)