FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_shrt_07r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_shrt_07r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15138236
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC248380.1640745989162806No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC240770.1590475931277594No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT213220.14084864313120762No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT163490.10799805208480037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT61200.030.8525641
ACTCTAG89950.023.8971255
GACTCTA88200.023.354254
CTAGCAG87450.022.012758
CTGACTC94000.020.2620282
CTCTAGC116600.017.8138076
TGACTCT117350.017.112033
TAGCAGA115150.017.0170529
TCTAGCA224200.016.9259937
GTGCGCA96950.015.62190168
GGTGCGC97850.015.44290667
TCTATCG127950.014.8039116
CTATCGA125800.014.75523217
TGCGCAT102650.014.68707769
GCGCGCC27850.014.49322162
AGCGCGC28550.014.37954361
AGGTGCG108600.013.9456166
CGGGAAT90900.013.3670061
GATCTAT143500.013.199594514
GCAGATC146950.013.17092311
Produced by FastQC (version 0.11.2)