FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_shrt_06r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_shrt_06r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53059730
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT1078770.20331238021753972No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC1050140.19791657439643964No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC1049260.1977507235713412No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT729010.13739421591478132No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT574250.1082270867190617No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTG553170.10425420559056747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT233950.034.303611
ACTCTAG338150.025.742635
GACTCTA339500.025.244094
CTGACTC348700.023.2821562
CTAGCAG359400.022.7110658
CTCTAGC435050.019.533066
TGACTCT448650.019.1487673
TAGCAGA436400.018.8145529
GTGCGCA443200.017.81824568
GGTGCGC449800.017.64111567
TCTAGCA933800.017.5188527
TGCGCAT464150.017.10315769
AGGTGCG486100.016.34440866
CTATCGA520200.015.36741317
TCTATCG527550.015.20570116
AAGGTGC568700.014.152513565
TGCGCTT570500.013.9868457
GATCTAT576300.013.94331214
GCAGATC581200.013.90226211
AGCAGAT603350.013.7744310
Produced by FastQC (version 0.11.2)