HTWKVBGX2_n01_shrt_05r.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HTWKVBGX2_n01_shrt_05r.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12623961
Sequences flagged as poor quality	0
Sequence length	75
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG	33395	0.26453662206339196	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT	25410	0.2012838917990954	No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAGAGTAACCTAGATTGATCAAACA	20278	0.16063104123975033	No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC	17855	0.14143738245072207	No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT	17814	0.1411126032471108	No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT	14585	0.11553426060172398	No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA	12638	0.1001112091521829	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TACTCTA	10970	45.16425	4
ACTCTAG	11785	42.831345	5
TTGTACT	11880	41.20201	1
GTACTCT	12000	41.17264	3
TGTACTC	12045	41.019146	2
CTAGTTG	12015	40.973278	8
TCTAGTT	12385	40.333843	7
CTGTTAC	13250	40.01602	1
CTCTAGT	12560	39.694027	6
TAGTTGT	13585	36.441498	9
GTTACCT	14160	34.40489	14
TGTTACC	14340	34.11671	13
ACCTCTA	14525	33.707	17
GTTACTA	16175	32.571796	3
CTAATGC	14890	32.5544	21
ACTACCC	16255	32.51762	6
TACCTCT	15060	32.509575	16
TGTTACT	16250	32.506653	2
TTACCTC	15285	31.940353	15
CCTCTAA	15600	31.405613	18