HTWKVBGX2_n01_shrt_04r.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HTWKVBGX2_n01_shrt_04r.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41473185
Sequences flagged as poor quality	0
Sequence length	75
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC	94908	0.22884184081835046	No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT	80317	0.1936600721647011	No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC	67959	0.1638625053754613	No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG	60614	0.14615226682011523	No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT	52386	0.12631294172367039	No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT	43759	0.10551154920944701	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ACTCTAG	39235	37.79582	5
GCTGACT	19385	37.19822	1
TACTCTA	21105	35.279194	4
CTGTTAC	30840	34.02583	1
ACTACCC	31585	32.83667	6
CTAGTTG	22815	32.359	8
TCTAGTT	24940	30.929579	7
GTTACTA	34325	30.265844	3
TGTACTC	24505	30.257988	2
TACTACC	34415	30.126432	5
CTCTAGT	25385	29.901745	6
TACCCTA	35250	29.301813	8
TTGTACT	25170	28.826971	1
GACTCTA	26770	28.496593	4
GTACTCT	26080	28.37738	3
CTACCCT	36590	28.322798	7
TTACTAC	36925	28.162766	4
TAGTTGT	26570	27.863943	9
CTGACTC	26025	27.814615	2
TGTTACT	38395	27.219677	2