FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_shrt_03r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_shrt_03r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17038725
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC447270.2625020358037353No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT425700.24984263787343244No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC417650.24511810596156697No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT296020.1737336567143375No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT244680.14360229418574452No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTTGGTCACCTTCAGCTTGGCGGTCTG234190.1374457302409658No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT205830.12080129235021986No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCGTC199710.11720947430045381No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA177200.10399839189845483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT83950.040.869771
GACTCTA123450.029.1511974
ACTCTAG127600.028.392385
CTGACTC128000.027.145032
CTAGCAG132550.026.4691128
TGACTCT156600.022.583713
CTCTAGC159050.022.4310726
TAGCAGA165300.021.3919539
TCTAGCA377650.017.9776337
GTGCGCA183250.017.07563268
GGTGCGC186150.016.80961267
TGCGCAT187650.016.69357969
CTATCGA206700.016.57487517
TCTATCG210750.016.3709516
AGGTGCG197650.015.86610566
GCAGATC221850.015.70630911
AGCAGAT229250.015.3645910
GCGCGCC49650.015.35575562
GATCTAT229100.015.08977914
AGCGCGC51450.014.75151961
Produced by FastQC (version 0.11.2)