FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_shrt_02r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_shrt_02r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14891598
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC241910.16244730753543038No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT224570.1508031575926237No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC213610.1434433027268128No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT152620.102487322045626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT62400.030.590821
ACTCTAG90550.023.8524885
CTAGCAG84100.022.848648
GACTCTA91950.022.5887724
CTGACTC91800.020.7843322
CTCTAGC115700.018.1904876
TAGCAGA113950.017.1357569
TCTAGCA223800.016.987127
TGACTCT120250.016.7563153
GTGCGCA98250.016.74977168
GGTGCGC99600.016.52262967
TGCGCAT105450.015.73693169
CTATCGA123150.015.38093917
AGGTGCG109650.015.10197566
TCTATCG129500.014.68002316
GATCTAT141150.013.32163714
CGGGAAT91750.013.0925361
GCAGATC146850.012.96836611
GGGGGCC63650.012.57462739
ATCTATC150850.012.55655415
Produced by FastQC (version 0.11.2)