FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_shrt_01r.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_shrt_01r.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42090433
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC938750.22303168038209537No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT782740.18596625033531966No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC758350.18017158435979977No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT561710.13345312936077422No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT491710.11682227170245552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT196400.035.853791
ACTCTAG303450.029.4139525
GACTCTA280100.026.6433964
CTGACTC281450.025.1061422
CTAGCAG287450.024.6273618
TACCCTA86300.021.0669988
TGACTCT355850.020.9621033
CTCTAGC357450.020.8585246
TAGCAGA356500.020.03169
ACTACCC98350.018.9787246
TCTAGCA753700.017.494117
CTATCGA410100.017.09506617
TCTATCG423050.016.61248816
CTAGTTG98200.016.3710778
CTACCCT117350.015.698497
GCAGATC460950.015.38822711
GATCTAT457600.015.27531814
CCTACAC130200.015.18318811
GTGCGCA366100.015.10610968
GGTGCGC374150.014.817924567
Produced by FastQC (version 0.11.2)