FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11689239
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT278790.23850141142635548No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA144300.12344687280326802No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC130060.11126472818290395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAC133550.016.8793531
GCAAGGG60200.016.2857721
CAAGGGC59900.015.8983232
GGGCGCC79800.014.61202466
TGCGCGC6950.014.39555469
AGGGCGC81300.014.38492565
GGGGCCC2404.010719E-614.37487169
GCGCCGT84400.013.93885668
TGGCCGC89000.013.567720
GCGCGTT67150.013.40918235
AGCGTCG92200.013.39541410
GCGTCGT90250.013.26481411
GATAACA172500.013.121512
CAGCGTC95750.013.0067489
AGTGGCC93400.013.00285918
CAGGGCG89150.013.00213164
CGTAGTG94150.012.97295915
CGGCGCG71750.012.74193733
TCGGCGC71800.012.73317232
ACATGGC176500.012.4328396
Produced by FastQC (version 0.11.2)