FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13024063
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT417400.3204837077339076No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA245190.18825922448317395No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC178370.13695419010181384No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA166110.1275408449728783No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG164410.12623556873150873No Hit
GATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTC159410.12239652096277484No Hit
GCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAG134270.10309378878158068No Hit
CCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGG133070.10217241731708454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG85250.022.3509861
CAAGGGC87700.021.5594582
GGATAAC203300.016.6395151
GCGAGGA121150.016.0033667
CGAGGAG142650.013.7848358
GATAACA247550.013.6869112
TAACATG251200.013.3366914
ATAACAT254600.013.1450383
ACATGGC257100.013.1245766
CATGGCC253600.013.0865247
CGTGGCC75800.012.92565660
CGTTCAC76100.012.69331966
GGCCGTT76500.012.49170963
AGCGTCG125500.012.47993410
GGGCGCC115150.012.46324766
AGGGCGC117600.012.4090465
CACGAGT262200.012.39467268
GCGCGTT101200.012.34070335
ATGGCCA270300.012.2779968
CGCGTAT19400.012.27721
Produced by FastQC (version 0.11.2)