FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10442289
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT235260.22529543091557802No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA143160.1370963780067761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGGGC53950.020.5295012
GCAAGGG56050.020.075721
GGATAAC117150.014.58451751
GCGAGGA79600.013.9985657
CGGGCAT9300.012.9901511
CGCGTAT11100.012.4384471
CGTGGCC44250.012.31817160
CGAGGAG94350.012.0296878
GGCCGTT45350.012.01932563
AAGGGCG96100.011.7404013
TGCGCGC5056.0936145E-1011.61391569
GATAACA150750.011.5126542
TAGGGGC54950.011.4267534
GCCGTTC47750.011.41521364
AGGGGCG54600.011.3735735
GCGCGTT58950.011.35363435
CGTTCAC50200.011.27048366
ATAACAT154000.011.1575063
CATGGCC148150.011.1306457
TCGGCGC61350.011.02200332
Produced by FastQC (version 0.11.2)