FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14073512
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC460660.3273241249234733No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT284540.20218123237469082No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC273910.1946280359870372No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT212650.15109945548772757No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT156500.11120180947015927No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT146880.10436627332253669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT87650.039.6583371
ACTCTAG106950.033.389485
CTGACTC106650.032.5780332
GACTCTA110000.032.150024
CTAGCAG117800.029.8130428
TAGCAGA135950.025.9598249
TGACTCT138300.025.9456393
CTCTAGC137950.025.6111056
CTATCGA166350.020.69844817
TCTATCG166350.020.69830116
GATCTAT179800.019.18833414
AGATCTA181250.019.09179513
TATCGAT182900.018.93842518
ATCTATC185100.018.69489515
GCAGATC188400.018.58645811
TCTAGCA300150.018.4704177
AGCAGAT193450.018.29717310
TAGCATG205700.016.60446227
ATTCTAG209000.016.44115323
CTAGCAT209400.016.34407626
Produced by FastQC (version 0.11.2)