FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9262830
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT212120.22900128794331753No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA124000.13386837499986504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG49800.019.6829811
CAAGGGC50450.018.8070642
GGGGCCC1605.763285E-415.09382869
GCGAGGA69450.014.6039597
GGATAAC104650.014.3796771
GGGCGCC60700.013.24242366
AGGGCGC62000.012.90918665
GCGCCGT65250.012.53116768
GCGCGTT54300.012.3258335
AAGGGCG80800.012.1269563
AGCGTCG70750.011.94647810
CAGGGCG68450.011.74309964
TGGCCGC70750.011.65460420
CGGCGCG56850.011.65164933
TAACATG126800.011.6457374
GACCGGC37950.011.6368645
CGTAGTG73450.011.461534515
TCAGCGT75100.011.4382528
CGAGGAG90100.011.4101598
TCGGCGC59000.011.40247832
Produced by FastQC (version 0.11.2)