FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13780651
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT456520.3312760768703888No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA232250.16853340237699946No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC205240.14893345749776263No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG188540.13681501693933038No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA184240.13369469990931487No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG182880.13270780894168208No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA181120.1314306559247455No Hit
GATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTC179230.13005916774178522No Hit
CCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGG171480.12443534053652473No Hit
GCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAG155850.11309335095998005No Hit
ATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCG150860.10947233189491556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG83050.021.9859281
CAAGGGC88250.020.7210542
CGTTCAC85150.018.59650866
CGTGGCC85550.018.54981860
GGCCGTT87650.017.90858563
GCCGTTC88550.017.76552664
GTTCACG89750.017.5670767
CCGTTCA89000.017.52071465
GGATAAC205700.016.7633171
TCGTGGC98950.016.03776459
TGGCCGT99900.015.81618862
GCGAGGA123500.015.1402337
TTCACGG108600.014.77204768
TCACGGA93450.014.76714969
GGGCGCC124850.013.48449566
GATAACA257550.013.476812
AGGGCGC126100.013.37818365
TAACATG257700.013.1073284
ACTCGTG119600.013.03789657
CGAGGAG145850.012.93841658
Produced by FastQC (version 0.11.2)