FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12429831
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT262920.2115233907846374No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA162070.13038793528246684No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC134280.10803043098494258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG63250.019.7031971
CAAGGGC63050.019.372372
GGATAAC141000.015.0327791
AGGGCGC82000.013.46298265
GCGAGGA91800.013.45367
GGGCGCC81700.013.42791366
CGCGTAT16250.013.1712921
GCGCCGT87650.012.67427468
CAGGGCG90600.012.03267964
GATAACA181150.011.8472252
AGCGTCG97700.011.75853210
GCGCGTT73300.011.71936435
ACCCGCG17450.011.66499113
TGGCCGC96900.011.535604520
CGAGGAG112200.011.3765038
GCGTCGT99300.011.36093711
AGGGGCG71650.011.26735
ATAACAT184100.011.2448413
CAGCGTC104850.011.219829
TAGGGGC72050.011.2044534
Produced by FastQC (version 0.11.2)