FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12884308
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT453560.35202511458124097No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA229690.17827111863516457No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC188060.1459604970635598No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA183020.14204876195135976No Hit
GATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTC178220.13832329993974066No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG177910.1380826971848236No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG169290.13139238832229094No Hit
CCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGG158120.1227229277660857No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA154640.12002196780766185No Hit
GCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAG154450.11987450160303526No Hit
ATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCG149650.11614903959141615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG87300.019.9280451
CAAGGGC89250.019.252292
GGATAAC208700.016.9862081
CGTTCAC82100.014.49689166
GGCCGTT83650.014.4758363
CCGTTCA84100.014.35735165
GCGCGTT107550.014.24256935
CGTGGCC84600.014.19099560
GCCGTTC86600.014.02255264
CGGCGCG111450.013.99187433
GCGAGGA125850.013.8980967
TCGGCGC111450.013.74422932
GATAACA263300.013.6676772
GTTCACG88050.013.63527867
ATGCCGC114700.013.5950468
GGCGCGT114550.013.52286534
CATGCCG116950.013.3923337
TGCCGCC118550.013.328149
TAACATG263750.013.2256884
ATAACAT267750.012.9894973
Produced by FastQC (version 0.11.2)