FastQCFastQC Report
Thu 22 Jun 2017
HTWKVBGX2_n01_protestrpt_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTWKVBGX2_n01_protestrpt_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16799180
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT354100.2107840977952495No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA231300.1376852917820989No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC177190.10547538629861695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG87200.019.0403021
CAAGGGC90500.017.9567932
GGATAAC198650.014.52656651
CGTGGCC72050.013.35899160
GGCCGTT73050.013.1288963
GCGAGGA129650.013.0649757
GCCGTTC75650.012.86011764
CGTTCAC78250.012.168179566
CCGTTCA79200.012.06581865
GGGCGCC113600.011.93479966
AGGGCGC115750.011.80256465
AGGGGGG8250.011.7098195
GATAACA254700.011.460352
GTTCACG87100.011.3679367
GCGCCGT123050.011.21491768
TGGCCGT88950.011.20870362
GCGCGTT97500.011.18123535
ATAACAT256800.011.1514613
TCGTGGC88800.011.14993759
ACTCGTG100600.011.07700168
Produced by FastQC (version 0.11.2)